Efficient protein purification independent from the protein class
Strep-Tactin®XT 4Flow® columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins.
The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets.
In addition, Strep-Tactin®XT 4Flow® allows the application of denaturing agents such as urea. In addition, a further increase in protein yield can be achieved through the high capacity variant of Strep-Tactin®XT 4Flow®.
- Higher affinity and protein yield even for large proteins.
- Higher capacity for large proteins.
- Broad pH stability (pH 4-10).
- Higher pressure stability compared to Strep-Tactin® Sepharose.
- Cost efficient.
Also available is the Strep-Tactin®XT 4Flow® Starter Kit, containing all reagents required for purification and detection of Strep-tagII® and Twin-Strep-tag® fusion proteins. See Starter Kit data sheet for more information.