Dyes & Stains – Ethidium Bromide Solution (Fisher Biotec)

Dyes & Stains – Ethidium Bromide Solution (Fisher Biotec)

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Ethidium Bromide Solution (EtBr) is a commonly used fluorescent stain to visualize nucleic acid
EtBr intercalates between DNA bases. Upon intercalation, its fluorescence increases several folds (25 fold increase when it binds DNA), much higher than the unbound ethidium bromide fluorescence. EtBr can detect as little as 1 ng DNA/band in agarose gel. It can also bind to RNA which results in 21 fold increase in its fluorescence intensity.