Cell Isolation – Fab-TACS® Gravity Columns (IBA LifeSciences)

Cell Isolation – Fab-TACS® Gravity Columns (IBA LifeSciences)

iba-lifesciences
Fab-TACS® Gravity - Get highly pure cell populations within a few convenient steps
The Fab-based traceless affinity cell selection (Fab-TACS®) technology utilizes a non-magnetic and fully reversible column-based immune affinity chromatography for cell isolation. The Fab-TACS® Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, whole blood or other blood preparations pass through the column. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. Advantages include:
  • High purity
  • High yield
  • High viability
  • Label-free cells
  • Directly from whole blood
  • No centrifugation.