EvaGreen® Dye is a next-generation DNA-binding dye with features ideal for use in quantitative real-time PCR (qPCR) and many other applications
EvaGreen® dye is designed using a novel concept of DNA binding via "release-on-demand" mechanism. The dye is constructed of two monomeric DNA-binding dyes linked by a flexible spacer. In the absence of DNA, the dimeric dye assumes a looped conformation that is inactive in DNA binding. EvaGreen® master mix can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition, making the mix suitable for both qPCR and HRM applications.
EvaGreen® Dye can be used as a general dsDNA-binding dye for DNA quantitation in solution, capillary gel electrophoresis and much more.
- Environmentally safe
- Superior for qPCR and isothermal amplification
- Unrivaled DNA melt curve performance
- Serving both as qPCR dye and a DNA gel stain
- Compatible with multiplex PCR
- Extremely stable
- Applicable in other applications.