Dyes & Stains – qPCR Master Mixes – EvaGreen® (Biotium)

Dyes & Stains – qPCR Master Mixes – EvaGreen® (Biotium)

biotium
EvaGreen® Dye is a next-generation DNA-binding dye with features ideal for use in quantitative real-time PCR (qPCR) and many other applications
EvaGreen® dye is designed using a novel concept of DNA binding via "release-on-demand" mechanism. The dye is constructed of two monomeric DNA-binding dyes linked by a flexible spacer. In the absence of DNA, the dimeric dye assumes a looped conformation that is inactive in DNA binding. EvaGreen® master mix can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition, making the mix suitable for both qPCR and HRM applications. EvaGreen® Dye can be used as a general dsDNA-binding dye for DNA quantitation in solution, capillary gel electrophoresis and much more.
  • Environmentally safe
  • Superior for qPCR and isothermal amplification
  • Unrivaled DNA melt curve performance
  • Serving both as qPCR dye and a DNA gel stain
  • Compatible with multiplex PCR
  • Extremely stable
  • Applicable in other applications.