Protein Purification – Strep-Tactin®Sepharose® (IBA Lifesciences)
Protein Purification – Strep-Tactin®Sepharose® (IBA Lifesciences)
The Strep-Tactin® Sepharose® resin is used for gravity flow purification of recombinant Strep-tag® proteins
Strep-Tactin® Sepharose® bulk resin is recommended for packing of columns since the resin is optimized for column affinity chromatography rather than for batch purification.
Binding capacity:
1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) is useful for the one-step purification of 50 to 100 nmol recombinant Strep-tag® protein (up to 3 mg in case of a 30 KDa protein).
