Protein Detection: Western Blotting – ECL Substrate Kits (Abcam)

Protein Detection: Western Blotting – ECL Substrate Kits (Abcam)

ECL Blotting Substrate Kit
The ECL Western Blotting Substrate (ab65623) is a sensitive, non-radioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) or immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
ECL Substrate Kit - High Sensitivity
The High Sensitivity ECL Substrate (ab133406) is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.
ECL Substrate Kit - Very High Sensitivity
The Very High Sensitivity ECL Substrate (ab133408) uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging. It is designed for the detection of proteins from 4.6pg to 4.7ng of protein per band. ECL Substrate Kit ab133408 produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins.
ECL Substrate Kit - Ultra High Sensitivity
The Ultra High Sensitivity ECL Substrate (ab133409) is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.2pg-2ng of protein per band. ECL Substrate Kit ab133409 produces a strong, long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins.
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