Protein Purification – Strep-Tactin®XT 4Flow® (IBA Lifesciences)

Protein Purification – Strep-Tactin®XT 4Flow® (IBA Lifesciences)

The Strep-Tactin®XT 4Flow® Starter Kit provides all you need for recombinant protein purification and Western Blot detection
Strep-Tactin®XT is a further engineered Strep-Tactin® variant with improved binding affinity for Strep-tag®II and Twin-Strep-tag®. It is suitable for purification of all protein classes, especially enzymes, metallo enzymes, membrane proteins, low abundant proteins and large proteins, without interfering with protein folding or enzyme activity. The Strep-Tactin®XT Starter Kit consists of:
  • 1 ml Strep-Tactin®XT 4Flow® gravity flow column
  • 1x Buffer W (Strep-Tactin®/Strep-Tactin®XT Wash Buffer)
  • 1x Buffer BXT (Strep-Tactin®XT Elution Buffer with Biotin)
  • 1x Buffer XT-R (Strep-Tactin®XT Regeneration Buffer)
  • Strep-Tactin® conjugated with HRP (for Western Blot Detection)
  • Short Protocol.
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