Electroporation & Electrofusion


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World leaders in electroporation technology supplying a complete range of electroporation equipment and accessories.

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Electroporation Systems - Agile Pulse Series

For maximum efficiency DNA vaccination delivery, the AgilePulse™ In vivo System is well suited for applications requiring high doses and robust immune responses such as gene therapy and cancer vaccines. Includes a user friendly programmable waveform generator and miniature parallel-needle electrode array.

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For large-volume applications of 2 to 20 ML, the AgilePulse MAX™System maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing. Transfection protocols can readily scale-up from standard laboratory cuvettes to large-volume transfection.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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Electrofusion Systems - Hybrimune Fusion Series

The Hybrimune™ System is an advanced electrofusion system for fast, efficient cell fusion in hybridoma production, hybrid cell formation or nuclear transfer applications.

The Hybrimune™ system includes an innovative fusion chamber design, proprietary BTXpress Cytofusion® medium and sophisticated, tri-phasic electric field pulses that quickly position cells and disrupt cell membranes for maximum cell fusion efficiency with short cycle-times and minimal heating or turbulence for excellent cell viability.

Applications include Hybridoma production; Dendritic and tumor cell fusion; and Nuclear transfer.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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Electroporation Systems - Gemini Series

The multi-waveform Electroporation System Gemini X² system is designed for researchers who need ultimate experiment flexibility. Electroporation of suspension cells can be achieved in cuvettes and 96 well plates. Suitable for In Vitro Cuvette/96 well applications, In Vivo, In Utero, In Ovo and Adherent Cell.

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>> View the Gemini X2 Electroporation System product video 

The Gemini SC2 system has a wide range of pulsing parameters, advanced safety features as well as dozens of pre-set protocols. Suitable for any lab requiring efficient cell transfection or transformatiion without the use of costly reagents.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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Electroporation Systems - ECM Series

The square wave pulse generator ECM® 830 is a Square Wave Pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo,  ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

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The ECM® 630 is an exponential decay wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying transfection applications. It features the ability to select the resistance and capacitance values, and adjust the range of voltages needed for efficient transformation of prokaryote and eukaryote transfection. The ECM 630 also features a flexible plug and play system to transition between standard cuvettes and to 25/96-well plates using the high throughput plate handler. ECM 630 applications include Transformation of Bacteria and Yeast; Transfection of Mammalian Cells; Transfection of Plant Tissue & Plant Protoplasts and High Throughput 25/96-Well.

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The ECM® 399 Electroporation System is specifically designed to produce the precise field strengths and pulse lengths required for the transfection of mammalian and transformation of bacterial and yeast cells. ECM 399 applications include Bacteria; Yeast and Fungus.

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Electrofusion Systems - ECM Series

The ECM® 2001 is a multi-purpose Electro Cell Manipulation generator capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Applications include Cell Fusion; Nuclear Transfer; Embryo Manipulation; Hybridoma Production; Mammalian Cell Transfection; Plant Protoplast Fusion and Stem Cell Production.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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HT Electroporation Systems - AgilePulse and HT systems

The High Throughput (HT) Systems offer multi-well electroporation technology for processing multiple samples in seconds. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96 well or 25 well electroporation plate by using one simple plate handler.

The HT Systems (ECM 830 or ECM 630) are comprised of 3 components - the HT Multi-Well Plates, an HT Plate Handler and the BTX ECM 830 or ECM 630 Generators. The HT plate handlers are compatible with the ECM 830 and 630 and some older ECM 830 and 630 generators.

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For efficient transfection for 50 to 1000 ML volumes, the newly released AgilePulse™ HT System is specifically engineered for high throughput volume applications and uses automated batch processing of smaller volumes ensuring uniform electroporation with minimal sample heating. Patented Pulse Agile® technology combines a unique sequence of short high-intensity pulses to porate cell membranes, followed by long low-intensity pulses to further drive transfectants into cells via electrophoresis, while maintaining cell viability.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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Microinjection Systems - Microjet Series

The MicroJect 1000 MAX (MJ 1000) is an alternative method to deliver genes, proteins, macromolecules and micro-beads by direct injection into cells, tissues and oocytes simply and efficiently. This microinjection system provides a reliable, consistent and precise delivery of volumes through stable pressure regulation maintaining this pressure for a set duration of time, controlled digitally.

The compressed gas internally controlled pressure system, allows the precise delivery of desired volumes ranging from femtoliters to microliter volumes. The MJ 1000 MAX is capable of holding a cell, oocyte or early stage embryo stationary while simultaneously using a separate pressure channel for injections. The MJ 1000 MAX is versatile enough to provide the same consistent performance needed to inject large volumes into tissue such as capillaries, or pico volumes, for nuclear injections. It is also ideal for the gentle transfer of delicate fetal or stem cells into oocytes.

To browse the BTX extensive line of products, services and accessories view the Catalog.

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Electroporation Cuvettes - Cuvetttes Plus

Cuvettes Plus™ are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation.

The cuvettes are molded with embedded aluminum electrodes, washed prior to packaging and gamma irradiated for guaranteed sterility. Three electrode gap sizes are available, 4mm, 2mm and 1mm for high field strengths up to 25.0 kV/cm. Round caps are leak resistant and allow for quick and easy one finger removal. BTX disposable Cuvettes Plus require no maintenance.

Cuvettes are compatible with most electroporation systems.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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Speciality Electrodes

An extensive range of Speciality Electrodes are available including the following:

  • Genetrodes™ for In Vivo, Ex Vivo & In Ovo Electroporation
  • Genepaddles™ for In Vitro Embryo & In Vivo Gene Delivery
  • Tweezertrodes™ for In Vivo Drug-Gene Delivery
  • 2-Needle Array™ for In Vivo Muscle Gene Therapy
  • Flatpack chambers for Stem Cell Transfection, Bacterial Transformation
  • Flat Electrode for Cell Fusion & Mammalian Cell Transfection
  • Caliper Electrodes for In Vivo Transdermal/Muscle Gene & Drug Delivery
  • Petri Pulser™ & Petri Dish Electrode
  • Microslides for Cell Fusion & Hybridoma Production
  • Meander Fusion Chamber for Cell Fusion
  • Platinum Needle L-Shaped Electrode
  • Petri Dish Platinum Electrode for Tissues
  • Petri Dish Platinum Electrode for Tissue Slices
  • Cables & Adapters.

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To browse the BTX extensive line of products, services and accessories view the Catalog.

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Reagents for Electroporation - BTXpress Buffers

BTXpress® electroporation buffer is a high performance, single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells. This solution, in combination with BTX electroporation instruments, provides researchers with the versatility and success needed across a broad range of cell types while maintaining critical cell viability.

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BTXpress Cytoporation® Media T and Media T4 are advanced electroporation buffers designed for use with the BTX AgilePulse MAX Large-Volume Electroporation System for ex vivo in vitro delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially-formulated to minimize heating of solution during large volumes electroporation for maximum transfection efficiency and high cell viability.

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View the BTXpress® brochure for more detailed information.

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