Cloning & Protein Expression
Product List:
Fisher Biotec
Our company specialises in the manufacture and distribution of leading-edge products for Molecular Biology, Genomics and Proteomics research.
Cloning Vectors
Fisher Biotec supplies a range of Cloning Vectors which include pSP64, PSP64(PolyA), pSP65(PolyA), & pSP71; pUC19; Lambda gt10 & Lambda gt11; and EMBL-3, EMBL-4 Vectors.
Biochemicals
Fisher Biotec supplies a range of Biochemicals which include Isopropyl-beta-D-Thiogalactopyranoside (IPTG), 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal), Dithiotreitol (DTT, Clelands Reagent), 5-Bromo-4-Chloro-3-Indolyl-beta-D-Glucoronide Cyclohexyl-ammonium salt (X-Glucuro-CHA salt), 4-Methylumbelliferyl-beta-D-Glucuronide (MUG).
Axygen Biosciences
Manufactures a comprehensive family of nucleic acid purification kits for life science research.
Staining Kits
The Axygen Ultra Fast Protein Stain Kit is a ready-to-use protein stain. The single step staining of proteins in polyacrylamide gels saves time and fast-bands start appearing in 3 minutes.
The Rapid Silver Staining Kit is a complete system for rapid and sensitive staining of proteins, DNA and RNA in polyacrylamide gels in 10 minutes.
Smart Ampicillin
Smart Ampicillin™ from Axygen Biosciences is a specially formulated Ampicillin antibiotic that is optimized for expression studies. Smart Ampicillin is superior to common antibiotics like Ampicillin, Carbenicillin and Methicillin and is specially formulated to overcome bacterial enzyme degradation.
X-Gal-IPTG Spray
X-Gal-IPTG Spray is a convenient ready to-use solution of optimal quantities of IPTG, X-Gal and stabilisers. X-Gal-IPTG spray can be directly sprayed on agar plates containing appropriate antibiotics. Spray solution is sufficient for approximately 100 plates (100 mm size).
KOMABIOTECH
Manufactures and provides reagents and tools for life science research including electrophoresis systems & pre-cast gels, PCR amplification reagents, nucleic acid purification kits, DNA methylation detection kit & ELISA kits.
Pre-Cast Gels
The extensive range of KOMA Pre-cast Gels include:
Protein Pre-cast Gel Complete Kit - choice of EzWay or Tris-Glycine gel (standard type of gel).
EzWay PAG - pre-cast SDS-PAGE system for protein analysis.
Tris-Glycine-PAG - pre-cast gel for most protein analysis.
Tricine-PAG - pre-cast gel for small proteins and peptide analysis.
Zymogram-PAG - pre-cast gel for identifying specific active proteases or collagenases.
EzWay IPG DryStrip - 1D and 2D gel electrophoresis.
IEF PAG - pre-cast gel for determining the isoelectric point (pl) of soluble proteins.
TBE-PAG - pre-cast gel for analysis of dsDNA, oligos or PCR products.
For more information refer to the Pre-Cast Gel Migration Chart & Specification.
Immunolgy Enrichment Kits
The EzWay™ Phos-Pep Enrichment Kit provides highly selective enrichment of phosphopeptide from non-phosphorylated complex tryptic digest of proteins and facilitates the identification of phosphopeptide by mass spectrometry.
The EzWay™ Phos-Pro Enrighment Kit allows the detection of protein phosphorylation in gels with non-radiolabelling method (eg sharing with fluorescence dye) and enables quantitative comparison between cells.
Western Blotting
EzWay™ DAB Western Blot Kit features specific and accurate colorimetric protein detection using DAB. This kit contains all necessary reagents optimized for best result of western blotting. HRP and DAB colorimetric substrate produce a dark brown band at the site of reaction.
The EzWay™ DAB Substrate Kit contains DAB and Substrate Solution for colorimetric detection of peroxidase (HRP).
Protein Staining Solution
KOMABIOTECH supply 3 types of Protein Staining Solutions:
EzWay™ Protein-Blue Staining Solution (ready-to-use highly sensitive staining).
EzWay™ Protein-Quick Blue Staining Solution (microwave compatibility protocol).
EzWay™ Protein-Silver Staining Kit (rapid and easy method).
Protein Elution
EzWay™ PAG Protein Elution Kit is used to extract and purify proteins from denaturing polyacrylamide gel after SDS-PAGE separation. It uses a simple device - masher bar and masher tube - for homogenization. Instead of the conventional electro elution method, the interested protein can be recovered easily, rapidly and conveniently with EzWay™ PAG Protein Elution Kit without any special equipment requirements. Only a table-top microcentrifuge is required.
