Cloning and Protein Expression


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IBA Lifesciences

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IBA is one of the foremost providers of TAG-derived products and services for PCR, cloning, transfection, protein expression and purification to detection, immobilization, and T-cell isolation.

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Cloning System

 Cloning System - StarGate®

StarGate is a cloning system which allows a fast and convenient screening for the optimal expression features. It offers rapid and highly efficient subcloning of an arbitrary gene - initially cloned into a Donor Vector - to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate destination vectors.

  • Fast recombinant protein expression optimization
  • Systematic screening for different features
  • Flexible fusion of different genes
  • High throughput cloning

For more information about the StarGate® Technology and for a detailed overview please click here.

Other StarGate® products available include sets for Standard Entry Cloning, products for Transfer Cloning and Fusion Cloning. Please contact us for more information.

Download the brochure here

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Mammalian Expression System

MEXi (Mammalian Expression IBA)

MEXi is an economic mammalian expression system in which all components are geared to each other. It includes a well transfecting expression vector backbone (pDSG-IBA), a HEK293/EBNA cell line (MEXi-293E) adapted to suspension growth, MEXi Culture Medium and MEXi Tranfection Medium. Due to their low biotin content, the cell culture media are perfectly adjusted to the IBA Strep tag®protein purification technology.

The use of mammalian cells for the expression of recombinant proteins, particular of secreted proteins, offers many advantages e.g. accurate protein folding or complex glycosylation. For more information about the MEXi system and for a detailed overview please click here.

Download the brochure here

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Protein Production Services (Custom)

Custom service for recombinant protein production includes:
  • Cloning - of the desired DNA into an expression vector with selected features
  • Protein expression - of the desired expression construct in E. coli, HEK293 or CHO cells
  • Protein purification - via affinity tags (Strep-tag®, Twin-Strep-tag® and 6xHis-tag).

Further options can be provided on request. Please contact Fisher Biotec for further information on any of these services.

Download the brochure here

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Protein Purification System

Protein Purification System - Strep-tag®

Based on the highly selective and easily controllable interaction between the Strep-tag®II peptide and the blotin blnding site of a specially engineered streptavidln called Strep-Tactin®. Strep-tag®II binds Strep-Tactin® nearly 100 times tighter than streptavidln, but elutes under gentle physlological conditions.

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Protein Purification System (One Step)

Protein Purification System - One-STrEP

An extremely fast and efficient method to identify protein complexes. The One-STrEP system isolates protein complexes by a single affinity purification step on Strep-Tactin® Superflow®, including short washing only, enabling co-purification of weakly associated preys. Physiological buffers are used throughout the purification process and elution is performed with minute concentrations of biotin in the same buffer.

  • Only one tag, only one purification step
  • Fast and easy purification conserves even weak protein:protein-interactions
  • Good signal-to-noise ratio
  • Mild elution conditions conserve functional structures of protein complexes
  • Convenient and highly flexible bait cloning procedure available (StarGate®).

Download the brochure here

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Magnetic Beads

Magnetic Beads MagStrep 'type3' XT

MagStrep 'type3' XT beads have a high binding capacity combined with very low non-specific protein binding. Elution can also be performed by boiling in denaturing SDS gel loading buffer instead of using biotin, if the isolated protein does not need to be functional. 

A specific elution buffer for native elution is required - bufferBX - biotin elution buffer. This buffer has a high biotin concentration and enables a more efficient elution.

  • High binding capacity
  • Very low non-specific protein binding due to improved coating
  • Flexible elution conditions
  • High affinity for Strep-tag, due to new Strep-Tactin XT

View the MagStrep "type3" XT beads flyer >> Instruction Manual and Protocol

 Download the brochure here

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Reagent - Apoptosis Assay

Apoptosis Assay - FasL-Strep

FasL-Strep is a pure and stable trimeric reagent for one-step purification of FasL-Strep on Strep-Tactin Sepharose.

  • Defined trimeric structure
  • High stability
  • Efficient apoptosis induction
  • Easy handling (detection, immobilization, isolation) via Strep-tag®.

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Reagent - T-Cell Expansion

Reagent - Streptamers® for T Cell Expansion

Products available include Streptamer Reagents for T cell expansion; Streptamer kits for Antigen-specific stimulation of T cells and Streptamer kits for pre-selection of T cells prior to expansion.

  • Novel reagents for polyclonal expansion of T cells
  • Non-magnetic soluble protein complexes generated by multimerization of aCD3- and aCD28 Fab-Streps with a Strep-Tactin® multimer
  • Completely reversible reagents, can be removed from the cells by the addition of biotin.

View the product data sheets for more information.

Download the brochure here

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Resins - Gravity Flow Columns

Protein Purification System - Strep-Tactin® XT Superflow® and Strep-Tactin® gravity flow colums

Strep-Tactin®XT Superflow® gravity flow columns are used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. They are available in different formats (0.2ml; 1ml; 5ml and 10ml columns).

Prepacked Strep-Tactin® gravity flow columns for gravity flow purification of Strep-tag fusion proteins are available with different resin supports:

  • Strep-Tactin® Sepharose®
  • Strep-Tactin® Superflow®
  • Strep-Tactin® Superflow® high capacity
  • Strep-Tactin® Macroprep®

They are available in different formats (0.2ml; 1ml; 5ml and 10ml columns).

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Resins - High Capacity

**NEW** Protein Purification System - Strep-Tactin® XT High Capacity Resin

A SIMPLE ONE-STEP PURIFICATION RESULTS IN HIGH PURITY!

Strep-Tactin®XT high capacity is a new resin for protein purification via Strep-tag®II or Twin-Strep-tag®. Strep-Tactin®XT high capacity efficiently captures recombinant proteins in high yields and together with unbeatable price it is the favorable alternative to all other Strep-Tactin® resins.

  • Highest affinity available around affinity purification systems
  • Higher protein yields compared to all other Strep-Tactin® resins
  • Elution of concentrated protein due to small elution volumes
  • Cost efficient: low price for more protein

Strep-Tactin®XT high capacity is employed in an easy and straight forward purification cycle, which leads to highly pure proteins. As all Strep-Tactin® resins, Strep-Tactin®XT high capacity can be regenerated and re-used at least 3-5 times.

View the Applicaton Notes showing the superiority of Strep-Tactin XT Superflow high capacity over Strep-Tactin Superflow high capacity, with regard to protein yield and efficiency of binding.

Watch the short YouTube video tutorial about the benefits of Strep-Tactin® XT high capacity.

Download the brochure here

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