Cloning and Protein Expression


Product List:

Maestrogen

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Develops and offers a wide variety of products including DNA/protein ladders, transilluminators, imagers, and other innovative research tools.

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Gel Documentation System (LED and UV)

Gel Documentation System - Slider Imager (LED) Blue Light

  • Provides high sensitivity for DNA and protein gel photography
  • Also can be a gel photography imager and cutting gel instrument
  • Digital camera, 100% cark room for photography
  • LCD (rotatable) monitor
  • Easy to access data via WiFi transfer.

Slider Imager (UV) Imager - Ultra-Violet light

  • Gel photography imager and cutting in the same instrument
  • Digital camera, 100% dark room for photography
  • LCD (rotatable) LCD monitor
  • USB interface - transfer and print data.
Download the brochure here
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Spectrophotometer - Micro-volume

Micro-Volume Spectrophotometer - ProNano

ProNano micro-volume spectrophotometer is designed for quick and accurate quantification of protein analysis.

  • A micro-volume 2µl 'stand alone' unit (no PC required)
  • Only 10 minutes reacting time is required
  • Linearity reaches R square >0.99 fpr every assay
  • No calibration necessary
  • Storage of data can be accessed via USB port to PC.

Download the brochure here

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Ladders - Protein

Protein Ladder - AccuRuler RGB Prestained Protein Ladder (250 µl)

  • Sharp and stable
  • Two reference bands - red at ~75 kDa and green at ~25 kDa
  • Ready to use - no need to boil
  • 3 µl or 5 µl per loading for clear visualization during electrophoresis
  • 2 µl to 3 µl per well for general Western transferring.

Download the brochure here

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Membrane Filtration Products

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Manufacturers of the CelluSep range of dialysis membranes and dialysis accessories.

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Dialysis Accessories

Dialysis Accessories - Membrane Clamps, Filter Funnels, Tubing Bag Holders and Start-up Kits

  • Locking dialysis membrane clamps - available in 3 sizes
  • Filter Funnels - make loading and unloading dialysis tubing easy
  • Dialysis tubing bag holders - strong polypropylene holders suspend dialysis bags in containers
  • Dialysis start-up kit - handy accessories and two types of regenerated cellulose tubular membrane.

Download the brochure here

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Dialyzers

Micro Dialyzer - QuixSep®

QuixSep is a simple, low cost device which can use membranes right off the shelf to dialyze samples too small for dialysis tubing.

  • Economical and can be reused hundreds of times
  • Pre-cut, low cost membrane squares are available
  • Can be used up to 55mm wide
  • Pre-cut membrane squares in three MWCO’s are also available.
  • 45 mm diameter membrane surface ensures a fast and complete dialysis procedure.

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Membranes - Cellulose Tubular

Regenerated Cellulose Tubular Membranes - Cellu·Sep®

  • Made from cotton and treated with glycerin to prevent the pores from collapsing
  • Air dried to form a rigid membrane
  • Free dialysis accessories in evey kit
  • Symmetric pore structure allows small molecules to migrate in either direction.

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Membranes - High Grade

High Grade Membranes - CelluSep H1 (Prepared dialysis membranes)

  • Heavy-metal free
  • Packed wet and EDTA treated
  • Individual-use
  • Available in 50cm lengths or 10m and 5m continuous rolls.

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Fisher Biotec

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Our company specialises in the manufacture and distribution of leading-edge products for Molecular Biology, Genomics and Proteomics research.

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Biochemicals - Cloning

Cloning Biochemicals

  • Isopropyl-beta-D-Thiogalactopyranoside (IPTG)
  • 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal)
  • Dithiotreitol (DTT, Clelands Reagent)
  • 5-Bromo-4-Chloro-3-Indolyl-beta-D-Glucoronide Cyclohexyl-ammonium salt (X-Glucuro-CHA salt)
  • 4-Methylumbelliferyl-beta-D-Glucuronide (MUG).

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IBA Lifesciences

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IBA is one of the foremost providers of TAG-derived products and services for PCR, cloning, transfection, protein expression and purification to detection, immobilization, and T-cell isolation.

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Cloning System

 Cloning System - StarGate®

Offers rapid and highly efficient subcloning of an arbitrary gene - initially cloned into a Donor Vector - to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate destination vectors.

  • Fast recombinant protein expression optimization
  • Systematic screening for different features
  • Flexible fusion of different genes
  • High throughput cloning

For more information about the StarGate® Technology and for a detailed overview please click here.

Other StarGate® products available include sets for Standard Entry Cloning, products for Transfer Cloning and Fusion Cloning. Please contact us for more information.

Download the brochure here

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Mammalian Expression System

MEXi (Mammalian Expression IBA)

MEXi is an economic mammalian expression system in which all components are geared to each other. It includes a well transfecting expression vector backbone (pDSG-IBA), a HEK293/EBNA cell line (MEXi-293E) adapted to suspension growth, MEXi Culture Medium and MEXi Tranfection Medium. Due to their low biotin content, the cell culture media are perfectly adjusted to the IBA Strep tag®protein purification technology.

The use of mammalian cells for the expression of recombinant proteins, particular of secreted proteins, offers many advantages e.g. accurate protein folding or complex glycosylation. For more information about the MEXi system and for a detailed overview please click here.

Download the brochure here

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