Assay Kits & Development Tools


Product List:

MRC Holland

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Development and production of simple to use DNA diagnostic kits based on the proprietary MLPA technique.

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SALSA MLPA Test Kits

MRC-Holland offers an ever-growing range of MLPA® Probemixes. MLPA (Multiplex Ligation-dependent Probe Amplification) is a multiplex PCR method detecting abnormal copy numbers of up to 50 different genomic DNA or RNA sequences, which is able to distinguish sequences differing in only one nucleotide (1).

The MLPA technique is easy to use and can be performed in many laboratories, as it only requires a thermocycler and capillary electrophoresis equipment. Up to 96 samples can be handled simultaneously, with results being available within 24 hours. To view the complete overview of all SALSA MLPA Probemixes offered by MRC-Holland, click on this link.

Below are the SALSA MLPA products list:

The MLPA Troubleshooting Wizard can help you solve your problems in the MLPA peak pattern. The wizard can be a useful addition to your MLPA workflow. This online tool is available on the MRC-Holland website.

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MLPA Reagents, Stabilisers and Enzymes

View the complete list of MLPA® Reagents, Stabilisers and Enzymes including:

  • SALSA® Sample Stabilising Solution **NEW**
  • SALSA® MLPA® Reagent Kits - Contain all MLPA® Reagents (Probemix not included)
  • SALSA® PCR Reagents
  • SALSA® MLPA® Reference Kits - To be used with self-made MLPA® probes
  • Control Fragments
  • SALSA® Polymerase - Excellent substitute for Taq Polymerase in PCR reactions
  • Ligase-65 - Themostable Ligase

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Life Diagnostics

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Manufacturers of ELISA kits, purified biomarkers and antibodies for use in cardiovascular, musculoskeletal, inflammation and immunology research.

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SPARCL Kits **NEW**

SPARCL™ kits (Spatial Proximity Analyte Reasgent Capture Luminescence) allow measurement of biomarkers in biological samples using a single set-up, 30 minute, no-wash immunoassay. A great alternative to ELISA's.

A typical SPARCL™ assay requires no wash steps, involves a single incubation, and takes approximately 45 minutes, including sample preparation time. This contrasts with ELISA's that require 1-4 incubations, 1-3 wash steps and take 2-4 hours.

Download the brochure here

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Cardiac Biomarker ELISA Kits

Cardiac Biomarker ELISA kits are available for species including mouse, rat, rabbit, dog, pig, and monkey:

NOTE: The ultra-sensitive cardiac troponin-I kits can be used for measurement of this important biomarker in both serum and plasma. The Ultra-Sensitive kits are approximately four-fold more sensitive than our High-Sensitivity kits.

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Acute Phase Protein ELISA Kits

Acute Phase Protein ELISA kits are available for the measurement of Acute Phase Proteins including CRP, Haptoglobin, Hemopexin, Alpha-1 Acid Glycoprotein, Clusterin, Alpha-2-Macroglobulin and Serum Amyloid A (SAA) from species including mouse, rat, rabbit, dog, pig, cow, chicken, and monkey.

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Skeletal Muscle Biomarker ELISA Kits

Skeletal Muscle Biomarker ELISA kits are available for the detection of skeletal muscle troponin-C, troponin-I, myoglobin and fatty acid binding protein (FABP) in species including mouse, rat, rabbit, dog, pig, and monkey. Also available are skeletal muscle troponin-I (fast twitch isoform) ELISA kits for mouse, rat, and monkey.

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Immunotoxicity ELISA Kits

Immunotoxicity ELISA kits are available for biomarkers in mice, rats, chickens and monkeys. Anti-KLH, anti-SRBC, anti-titanus toxoid, anti-DNP and anti-TNP lgM and lgG are used as biomarkers of immunotoxicity.

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Immunoglobulin ELISA Kits

Immunoglobulin ELISA kits are available for the measurement of Immunoglobulin levels (IgA, IgE, IgG and IgM) in species including mouse, rat, guinea pig, rabbit. cat, dog, pig, monkey, camel, cow, chicken, and turkey. All assays are in a 96-well format. The total assay time is approximately 2.5 hours.

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Liver Biomarker ELISA Kits

Liver Biomarker ELISA kits are available for Vitamin D-binding protein (Gc globulin) and liver fatty acid binding protein (L-FABP). Both are biomarkers of liver toxicity and injury. Acute phase proteins (haptoglobin and hemopexin) have also been identified as biomarkers of liver damage and it is possible that other acute phase proteins, almost all of which are expressed by hepatocytes, may prove useful as markers of liver damage.

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PEG ELISA Kits

PEG ELISA kits are available for the measurement of methoxy-PEG and non-methoxy-PEG (free and conjugated) as well as ELISA kits for detection of anti-PEG IgM and IgG in mouse, rat and monkey serum or plasma.

Researchers should be aware that many commercially available ELISA buffers contain Tween-20 or other polyoxyethylene detergents that significantly interfere with PEG assays. Our blocking buffer is devoid of polyoxyethylene detergents and can be used to block and stabilize plates coated with PEGylated proteins or PEG antibodies.

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