Australia - Fisher Biotec

Biosearch Technologies offers a variety of Probes and Primers for real-time qPCR gene expression assays and SNP genotyping.

Dual-labeled BHQ® Probes are available in a wide selection of fluorophore-quencher combinations for dual-labeled probes. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

The BHQplus™ Probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye.

Molecular Beacons have short complementary sequences which hybridize to form a stem-loop structure that holds the fluorophore and quencher close together in space.

Scorpions® Primers combines the probe and primer into one molecule and uses a uni-molecular mechanism that does not require enzymatic cleavage of the probe during PCR cycling.

Calibration and Reference Dyes (CAL Fluor® and Quasar® dye) calibration standards are designed to improve the accuracy of sample signal in real-time thermal cyclers that require spectral calibration.

SuperROX® Reference Dye is a specially formulated version of the ROX (carboxy-X-rhodamine) dye.

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What is Stellaris RNA FISH?

Stellaris™ FISH (fluorescence in situ hybridization) is a RNA visualization method that allows simultaneous detection, localization, and quantification of individual mRNA molecules at the sub-cellular level in fixed samples using widefield fluorescence microscopy. A set of Stellaris FISH Probes comprises multiple oligonucleotides with different sequences each with a fluorescent label that collectively bind along the same target transcript to produce a punctate signal.

The Stellaris FISH technology follows a simple protocol, does not use exotic reagents, is inexpensive and platform-independent, offers same-day results, and is versatile toward many sample types and applications.

View the Protocol to learn more about the Stellaris FISH technique.

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